Posted by Administrator on July 17 2009 16:18
Introduction
Pigs may become infected via exposure to PRRSV by any of several routes:
intranasal
intramuscular
oral
intrauterine
vaginal
Aerosol transmission of PRRSV from infected to susceptible pigs has been demonstrated under experimental conditions. However, the inability to consistently infect susceptible animals under experimental conditions indicates that we do not fully understand the conditions essential for aerosol transmission of PRRSV
In general, aerosol transmission of ... [More]
Posted by Administrator on July 16 2009 14:44
Introduction
Swine respiratory disease is often complex with multiple viral and bacterial pathogens involved. The pig and sample selection process is very important in obtaining and making the diagnosis. Also, as more is known about swine respiratory pathogens and their various serovars, serotypes, strains, virulence genes, and mutations, it may now be necessary to delve more deeply into the diagnosis than before.
Pig selection
The selection of pigs for testing, whether for serology or necrop... [More]
Posted by Dr. Steve Dudley on June 29 2009 13:49
These two diseases have been the most economically important diseases recently.
Prevention and control strategies have been an area of focus in herd health.
We have come a long way in our understanding of these pathogens, but there is still much to learn and accomplish.
Porcine Reproductive and Respiratory Syndrome Virus
PRRS is the disease..... PRRSV is the virus
Several Other Names
SAMS
SMD
SIRS
Blue Ear
Lelystad
Most economically important disease of swine
... [More]
Posted by Administrator on June 19 2009 09:13
PRRSV has been reported to survive in well water and city water out to 9 and 11 days post-inoculation, respectively. No assessment has been made in regards to viability in lagoon water. At the request of one of our board members, I conducted the following small trial:
Objective: To determine the duration of PRRSV survivability in lagoon water.
Methods: Ten ml of water from a 1-stage re-circulation lagoon from a large swine production company was inoculated with 1 ml of PRRSV (strain MN 30-100,... [More]
Posted by Administrator on June 19 2009 09:02
Greetings! Below find new information on whether Aedes vexans can serve as a biological vector of PRRSV:
Experiment I: Assessment of the duration of survivability and location of PRRSV in mosquitoes following feeding on an infected pig.
Diagnostic results from Experiment I are summarized in Table 1. A set of 10 blood-fed mosquitoes was randomly collected at each of the following sampling times: View Image0 hour (h), 6 h, 12 h, 24 h, 48 h, 72 h, 5 days (d), 7 d, 10 d, and 14 d. PRRSV nucleic ... [More]
Posted by Administrator on June 19 2009 08:54
Update of data from PRRSV aerosol study
Hello,
Just so you hear this first, I have some new information regarding this study:
Five replicates were conducted over distances of 30-150 meters (100-500 feet).
PCR data were consistently reproducible:
5/5 PCR (+) @ 30, 60, 100 m
4/5 PCR (+) @ 120 m
3/5 PCR (+) @ 150 m.
Viable PRRSV has been isolated from air samples collected over the 150-m distance. (data from recent replicates still pending).
The temperature, humidity, and veloci... [More]
Posted by Dr. Steve Dudley on June 19 2009 08:11
Summary of Intervention Strategies for the Prevention of Mechanical Transmission of PRRSV during Cold Weather.
Background: The "snowball" study demonstrated the ease at which PRRSV could be mechanically transmitted by a number of different fomites during periods of cold weather. High-risk fomites and sites were determined to be truckwash floors, boots, vehicle interior (floormats), anteroom floors within swine facilities, and shipping containers.
Objective: The purpose of this study was to tes... [More]
Posted by Dr. Steve Dudley on June 18 2009 16:09
Evaluation of aerosol transmission of Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (mixed infection) under field conditions
Introduction
M. hyopneumoniae potentiation of PRRSV has been demonstrated, but it is not known if this can increase the probability of viral transmission.
The objectives of this study were to evaluate the transmission of M. hyopneumoniae and PRRSV by aerosol both as a single or as a mixed infection
Materials and Methods
A total of ... [More]
Posted by Dr. Steve Dudley on June 18 2009 13:54
Background
We have been testing the hypothesis that aerosols can transmit PRRSV over distances greater than 1 meter under controlled field conditions. In 2 attempts, we have not observed PRRSV transmission by aerosols originating from an infected finishing pig population to trailers of naïve pigs. Attempts to detect PRRSV from air samples collected in the infected animal airspace, from fan exhaust, or from air tubes using an all-glass impinger (AIG) have not been successful. Critical analy... [More]